The Molecular Physiology Laboratory is working on the structure – function relationship of signalling molecules, with a focus on voltage dependent sodium channels (vdsc’s) and G-protein activated potassium channels (GIRK's). Central topic is the regulation of ion channels by protein phosphorylation and G-proteins and subunit composition.
(i) cell culture: Xenopus laevis oocytes; cardiomyocytes; cell culture
(ii) genetic engineering: heterologous expression in bacteria, mammalian cells and Xenopus laevis oocytes; site directed mutagenesis; RNA isolation and synthesis; DNA constructs; production and high-scale purification of recombinant protein;
(iii) biochemistry: protein interaction using the pull-down assay; in-vitro phosphorylation (Phosphorimager);
(iv) cell biology: confocal laser scanning microscopy in living cells; protein-protein interaction in vivo using FRET; phosphorylation and back-phosphorylation in vivo in living cells;
(v) electrophysiology: two electrode voltage clamp of whole cells; patch clamp techniques.
2 rigs for Two Electrode Voltage Clamp on oocytes
1 rig for patch clamp experiments
1 rig for TEVC and patch clamp experiments simultaneous confocal LSM. Additional CCD camera system for conventional epifluorescent measurements work space for the preparation, injection and culture of Xenopus laevis oocytes
high capacity autoclave
shaker for incubation of bacteria
Ultra Sonic desintegrator for recombinant protein purification from bacteria High Speed Centrifuge
High capacity centrifuge
PCR Thermocycler Eppendorff PCR Gradient Thermocycler
Frog room within the animal facility of the preclinical building
Biofreezer all kind of standard laboratory equipment.